Ultrafast Characterization and Rapid Quantitation of Antibody Proteins Using Microdroplet and Coulometric Mass Spectrometry Approaches
Microdroplet has become an interesting media for acceleration of various reactions.
Recently, we reported an ultrafast microdroplet-based digestion approach for antibody
including mutated antibodies in which the digestion can complete within 1 ms. Such fast
digestion thus facilitates rapid and high throughput characterization of antibody protein
therapeutics by high resolution mass spectrometry. On the other hand, antibody proteins
can be also quantified by coulometric mass spectrometry (CMS), a combined
electrochemistry and mass spectrometry technique (EC/MS). CMS can be used to
perform absolute quantitation of small organic molecules, peptides and proteins as well
as protein post-translational modifications, without the use of standards. It is based on
Faraday’s Law. My talk will focus on the development and applications of microdroplet
mass spectrometry and coulometric mass spectrometry.
Hao Chen graduated from Wuhan University in 1996 and obtained his Ph.D. degree from
Purdue University in 2005. He is currently a professor in chemistry at New Jersey Institute
of Technology (NJIT). Dr. Chen performs research on organic and biological mass
spectrometry for bioanalysis and instrument development and has published 160+ papers in
peer-reviewed journals (H index 52).