Professor Rustem Ismagilov of California Institute of Technology

Professor Rustem Ismagilov of California Institute of Technology will present

Towards Rapid Antibiotic Susceptibility Testing Using Digital Single Molecule Counting

on November 11, 2017 at 2:10 PM in Neville Hall, Room 3.

Abstract:

Rapid antimicrobial susceptibility testing (AST) is desperately needed for informing treatment decisions and preventing the spread of antimicrobial resistance resulting from the misuse and overuse of antibiotics.  To date, no phenotypic AST exists that can be performed within a single patient visit (30 min) directly from clinical samples.  Here we show that AST results can be obtained by exposing clinical urine samples to an antibiotic for 15 minutes and using digital nucleic acid quantification to measure precisely that phenotypic response of the infecting pathogen.  We demonstrate this AST method using an ultrafast ~7 minute digital real-time loop-mediated isothermal amplification (dLAMP) assay (AUC=0.96).  We also demonstrate that the rapid digital LAMP assay can be used with SlipChips to determine the phenotypic antibiotic susceptibility of E. coli directly from clinical urine samples in less than 30 minutes sample-to-answer, including all sample processing and data analysis steps.