Seminar
Tuesday, November 13, 2018 - 12:00am
Gradute Student Jennie Cawley will present
"Surface Enhanced Raman Spectroscopy toward Medical Diagnostics"
on November 13, 2018 at 4:10 PM in Neville Hall, room 3
Enhanced Raman scattering techniques can be used to boost inherently weak signals from Raman scattering. This presentation will cover the recent advances in surface enhanced Raman scattering (SERS) and its applications in biological systems. The high sensitivity of the technique is made possible through the excitation of localized surface plasmons which amplify the local electromagnetic (EM) field and enhance Raman scattering signals.1 Manipulating the factors that increase the chemical and electromagnetic enhancement of Raman scattering have transformed the potential of SERS. The first objective of this presentation is to highlight the work of Willner, et al. in which glycan expression on cancerous prostate cells was detected using SERS in combination with droplet microfluidics.2 This paper highlights the power of SERS to detect different glycan biomarker expression levels on the surfaces of individual cells. Detecting upregulation of biomarker expression allows the classification of a cell as cancerous or non-cancerous, demonstrating the potential of SERS in diagnostics. The second part of the talk will illustrate the ability to detect and differentiate influenza viral strains from non-influenza and non-viral strains by SERS.3 The ability of SERS to rapidly and precisely detect and differentiate viruses is particularly important for preventing spread of viral infection by new influenza strains until vaccines are available. Lastly, I will discuss the work of Tsao, et al. in which SERS was used to map the phenotypic evolution of circulating tumor cells (CTCs) from human patients.4 Their multi-marker detection of CTCs has potential to provide clinicians with real-time monitoring of phenotypic evolution that results from targeted anti-cancer therapy. References (1) Sharma, B.; Frontiera, R. R.; Henry, A.-I.; Ringe, E.; Van Duyne, R. P. SERS: Materials, Applications, and the Future. Mater. Today 2012, 15 (1–2), 16–25. (2) Willner, M. R.; McMillan, K. S.; Graham, D.; Vikesland, P. J.; Zagnoni, M. Surface-Enhanced Raman Scattering Based Microfluidics for Single-Cell Analysis. Anal. Chem. 2018, 90 (20), 12004–12010. (3) Lim, J.; Nam, J.; Yang, S.; Shin, H.; Jang, Y.; Bae, G.-U.; Kang, T.; Lim, K.; Choi, Y. Identification of Newly Emerging Influenza Viruses by Surface-Enhanced Raman Spectroscopy. Anal. Chem. 2015, 87 (23), 11652–11659. (4) Tsao, S. C.-H.; Wang, J.; Wang, Y.; Behren, A.; Cebon, J.; Trau, M. Characterising the Phenotypic Evolution of Circulating Tumour Cells during Treatment. Nat. Commun. 2018, 9 (1), 1482.